The green synthesis of zinc oxide nanoparticles (ZnO-NPs) using plant extracts offers an eco-friendly and sustainable approach with promising applications in both biomedicine and environmental remediation. This study focuses on the synthesis of zinc oxide nanoparticles (MS-ZnO-NPs) using Mentha spicata extract and evaluates their antioxidant and antibacterial properties, positioning them as potential candidates for sustainable healthcare and wastewater treatment. The synthesis was carried out under optimized conditions, using a 10% extract-to-Zn precursor ratio and a reaction time of 120 minutes. The resulting MS-ZnO-NPs were characterized by ATR-FTIR, XRD, and SEM-EDX. The antioxidant activity of both the plant extract and MS-ZnO-NPs was evaluated and showed very good activity. Antibacterial efficacy was tested against 08 reference bacterial strains as well as bacteria isolated from industrial effluent, and the results demonstrated that both MS-ZnO-NPs and the plant extract possess good antibacterial activity. These findings highlight the antioxidant and antibacterial potential of Mentha spicata extract and its derived nanoparticles, supporting their applicability in biomedical fields and environmental solutions such as wastewater treatment